ief electrode strip pads  (GE Healthcare)

Journal: Planta

Article Title: Absence of photosynthetic state transitions in alien chloroplasts

doi: 10.1007/s00425-019-03187-2

Figure Lengend Snippet: Isolation of light-harvesting complexes. a Thylakoid protein preparations separated on the basis of pI by isoelectric focusing. Photographs of representative gels from tobacco (T), the Nt ( Hn ) cybrid and henbane (H). The boxed area near the cathode defines the site where thylakoids were loaded into the gel. The pH scale and distance from cathode are marked above and below the gel, respectively. The former is approximate as pH gradients varied slightly between gels. Images have been cropped to show the central aspect of the gel tray for easy comparison. b SDS–PAGE separation of LHCII polypeptides from the broad IEF band in a 15% acrylamide gel. The protein marker is shown in the first lane (m) the standard weights are indicated. The pink arrow indicates the novel band in the Nt ( Hn ) separation. c Density plots of the MW profiles shown in b . The plots from left to right correspond to the top to bottom of the gel in b , respectively. Tobacco is shown in green and the Nt ( Hn ) cybrid in blue. Peaks were numbered 1–7 in tobacco with equivalent peaks in Nt ( Hn ) density plots assigned letters ( A – H )

Article Snippet: Two IEF electrode strips (GE Healthcare) were saturated, one in anode solution (5.3% (v/v) H 3 PO 4 ) and one in cathode solution (1 M NaOH), and carefully positioned at the anode and the cathode positions, respectively.

Techniques: Isolation, SDS Page, Acrylamide Gel Assay, Marker